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Effects of diamide on CFTR mutants at the R-CL3 interface. A–C, macroscopic currents across inside-out membrane patches excised from transfected HEK-293T cells expressing CFTR mutants S768C/V510A (A), S832C/V510A (B), and S768C/S832C/V510A (C) by using a ramp protocol (±80 mV). NPPB-AM is a CFTR-specific opener (25). D, fractional inhibition of the current by diamide for three CFTR mutants (n = 3–5; *, p < 0.05, unpaired t test); error bars, S.E.
