FIGURE 7.

Inhibition of the Hsp90 chaperone complex reduces levels of the disease-causing I477N myocilin. A and B, Western blot of lysates from HEK iI477N cells treated with the indicated concentrations of the pan Hsp90/Grp94 inhibitors ¹⁷AAG (A) and celastrol (B) for 24 h. C, Western blot of lysates from HEK cells conditionally over-expressing I477N myocilin treated with 7.5 μm ¹⁷AAG or vehicle (Veh) and harvested at indicated time points. D, dot blot of the cell culture media of HEK iWT cells that were treated with the indicated concentration of ¹⁷AAG. E, Western blot of lysates from HEK iI477N cells and treated with indicated concentrations of a Grp94-selective inhibitor (Grp94 Sel. Inh.) for 24 h.