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Sdh1 is flavinylated in the absence of the membrane anchor subunits and forms a stable dimeric complex with Sdh2. A, steady-state immunoblot and Sdh1 flavinylation analysis (UV illumination). B, nickel-nitrilotriacetic acid purification of Sdh2-His6Myc2 in mitochondria isolated from sdh3Δsdh4Δ cells. UB, unbound; E, eluate; Flavo, flavoprotein.
