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. 2012 Sep 27;287(48):40690–40702. doi: 10.1074/jbc.M112.390419

TABLE 3.

Kinetic parameters of mAOX3 variants obtained at 37 °C, pH 8.00

ND, none detectable.

Substrate	Benzaldehyde^a			N1-Methylnicotinamid^a
Substrate	k_cat	K_m	k_cat/K_m	k_cat^b	K_m	k_cat/K_m
	min⁻¹	μm	μm/min	min⁻¹	μm	μm/min
mAOX3-WT	41.9 ± 0.8	2.5 ± 0.2	16.76	14.7 ± 0.1	128.5 ± 5.8	0.115
E1266Q	10.2 ± 0.5	86.3 ± 11.3	0.12	ND	ND
A807V	41.0 ± 0.3	2.9 ± 0.2	14.14	11.2 ± 0.1	123.4 ± 5.4	0.091
Y885M	57.4 ± 0.8	6.3 ± 0.3	9.11	23.4 ± 0.4	82.7 ± 6.6	0.283
A807V/Y885M	43.6 ± 1.1	4.6 ± 0.4	9.48	27.0 ± 0.2	35.9 ± 1.4	0.752
K889H	19.2 ± 0.5	2.3 ± 0.3	8.20	3.1 ± 0.1	25.5 ± 4.0	0.120

Substrate	Phenanthridine^b			Phthalazine^a
Substrate	k_cat	K_m	k_cat/K_m	k_cat	K_m	k_cat/K_m
	min⁻¹	μm	μm/min	min⁻¹	μm	μm/min
mAOX3-WT	51.7 ± 0.6	32.3 ± 1.4	1.60	41.1 ± 1.0	1.4 ± 0.2	29.36
E1266Q	ND	ND		ND	ND
A807V	315.0 ± 5.2	149.5 ± 5.8	2.11	41.1 ± 1.5	2.7 ± 0.4	15.22
Y885M	266.9 ± 66.5	33.4 ± 11.6	7.99	48.9 ± 1.2	3.2 ± 0.2	15.28
A807V/Y885M	218.0 ± 17.1	11.8 ± 1.7	18.47	41.4 ± 1.8	2.9 ± 0.3	14.28
K889H	79.9 ± 0.7	16.1 ± 0.6	4.97	11.8 ± 0.3	1.1 ± 0.2	10.53

^a As terminal electron acceptor 100 μm DCPIP were used.

^b As terminal electron acceptor molecular oxygen in air saturated buffer was used.