FIGURE 5.

Validation of the dFib-OCT4^GFP–ind reporter system. A, C, and E, GFP detection and NANOG/TRA-1-60 immunofluorescence analysis in dFib-OCT4^GFP–indSKC-2 (A), dFib-OCT4^GFP–indOKC-1 (C), and dFib-OCT4^GFP–indOSK-11 (E) cells infected with retroviruses encoding the missing factor and/or treated with doxycycline (DOX) for 18 days. Scale bar = 150 μm. B, D, and F, evaluation of reprogramming efficiency in dFib-OCT4^GFP–indSKC-2 (B), dFib-OCT4^GFP–indOKC-1 (D), and dFib-OCT4^GFP–indOSK-11 (F) cells infected with retroviruses encoding the missing factor and/or treated with doxycycline for 18 days. Reprogramming efficiency was evaluated as the number of Nanog+ colonies per 50,000 dFib-OCT4^GFP–ind plated cells on MEFs after retroviral infection.