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. 2012 Sep 25;287(48):40779–40792. doi: 10.1074/jbc.M112.411181

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Lens epithelial cells constitutively express FPR1. Western blots were probed in panels A and B and the top portion of C with NFPR2, a mAb to FPR1. PMN stands for polymorphonuclear leukocytes (neutrophils). The bottom portion of panel C depicts the blot from the top of panel C reprobed with anti-β-actin after stripping. Panel D depicts lanes 5–8 from the blot in panel C stained with Ponceau S as an additional loading control. The amount of membrane protein loaded is indicated below each lane. The cell types tested and cell treatments are indicated at the top. HEK hFPR1⁺, FPR1-transfected HEK 293 cells. Western blots in A, B, and C are from separate experiments. For A and B, the Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific) was used, and for C the chemiluminescent Amersham Biosciences ECL Plus detection reagent (GE Healthcare) was used.