TABLE 2.
Comparison of the pIC50/pKi values of fMLF, fNLFNYK-Fl, and peptide W at the FPR1 in FHL 124 cells, HEK 293-FPR1+ cells, and human neutrophils (PMN)
The data are the mean values ± S.E. from two independent experiments with duplicates.
| Ligand | FHL 124 |
HEK 293-FPR1+ |
PMN |
|||
|---|---|---|---|---|---|---|
| pIC50 | pKia | pIC50 | pKib | pIC50 | pKic | |
| fMLF | 6.80 ± 0.17 | 7.65 ± 0.17 | 6.81 ± 0.06 | 7.14 ± 0.06 | 6.67 ± 0.06 | 6.96 ± 0.06 |
| fNLFNYK | 7.91 ± 0.23 | 8.76 ± 0.23 | 7.82 ± 0.08 | 8.15 ± 0.08 | 7.74 ± 0.05 | 8.03 ± 0.05 |
| Peptide W | 7.33 ± 0.20 | 8.18 ± 0.20 | 7.55 ± 0.05 | 7.88 ± 0.05 | 7.17 ± 0.06 | 7.46 ± 0.06 |
a Calculated with a KD of 0.5 ± 0.2 nm.
b Calculated with a KD of 2.7 ± 0.3 nm.
c Calculated with a KD of 3.2 ± 0.1 nm.