Table 2. Sequences of PCR primers and probes.
Gene | FAM/TAMRA probe | Forward primer | Reverse primer | Amplicon length (bp) |
---|---|---|---|---|
TMSB15A | CAAGCTTTCAGGCTATCTTCTAGTCAA | CCGCGAACAGCCTTTCAC | CGACAAGTCTGGCTTATCACTCA | 77 |
ESR1 | ATGCCCTTTTGCCGATGCA | GCCAAATTGTGTTTGATGGATTAA | GACAAAACCGAGTCACATCAGTAATAG | 73 |
HER2 | ACCAGGACCCACCAGAGCGGG | CCAGCCTTCGACAACCTCTATT | TGCCGTAGGTGTCCCTTTG | 87 |
OAZ1 | TGCTTCCACAAGAACCGCGAGGA | CGAGCCGACCATGTCTTCAT | AAGCCCAAAAAGCTGAAGGTT | 83 |
CALM2 | TCGCGTCTCGGAAACCGGTAGC | GAGCGAGCTGAGTGGTTGTG | AGTCAGTTGGTCAGCCATGCT | 72 |
RPL37A | TGGCTGGCGGTGCCTGGA | CCGCGAACAGCCTTTCAC | CGACAAGTCTGGCTTATCACTCA | 77 |
Sequences are given in 5′ to 3′ orientation.