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Immunoperoxidase staining of frozen sections of metastases: (a) tumor cells from both progressing and regressing metastases do not express HLA-DR. The staining shows a high degree of HLA-DR positive cellular infiltration in regressing metastasis; in contrast, the progressing lesions have very little infiltration. (b) CD3 staining corresponds to T lymphocyte infiltration (c and d). The number of CD4 positive cells in the infiltrate is higher than the number of CD8 cells.
