Figure 4.

Cannabidiol affects the protein expression profile of HUVECs. (A) HUVECs were treated with CBD for 24 h and supernatants were used to determine different protein levels through a human antibody array kit/proteome profiler. Representative proteomic membrane analysis with the indication of proteins modified. (B) Densitometric analysis of the membrane spots reported as percentage of the untreated control. Data represent the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus Control, Dunnett's t-test. (C) Western blot analysis of 5B4 antibody against uPA. A representative Western blot is shown. NT, not treated.