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. 2012 Nov;167(6):1232–1243. doi: 10.1111/j.1476-5381.2012.02058.x

Figure 4.

Figure 4

Effect of 3-MFA or FFA applied intracellularly. (A) Whole-cell patch was recorded in the TRPM2 cells using pipette solution containing 0.5 mM ADP-ribose (ADP-r) with or without FFA (200 µM) or 3-MFA (200 µM) (n= 5 for each group). (B) Example of outside-out patches showing the effect of FFA (100 µM) and 2-APB (100 µM). (C) Example of the effect of 3-MFA (100 µM) and 2-APB (100 µM). (D) Single channel activity of TRPM2 recorded by outside-out patches (n= 4). (E) Mean unitary current sizes for ADP-ribose-induced TRPM2 single channel events plotted against voltage. Straight lines were fitted, and the mean unitary slope conductance was 65.76 ± 0.23 pS (0.5 mM ADP-ribose). No effect of FFA (100 µM, 65.81 pS) and 3-MFA (100 µM, 65.61 pS) on the single channel conductance in the outside-out patches.