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. 2012 Nov;167(6):1311–1328. doi: 10.1111/j.1476-5381.2012.02086.x

Figure 5.

Figure 5

Lysosomal and autophagosome markers in MELAS fibroblasts. (A) Cultured control and MELAS fibroblasts incubated with 0.06 µM riboflavin or 100 µM CoQ were fixed, and immunostained with anti-LC3 (autophagosome marker) and cytochrome c (mitochondrial marker) and examined by fluorescence microscopy. A representative picture of the 100 cells analysed is shown. Colocalization of both markers was assessed by the DeltaVision software. (B) Magnification of a small area of a MELAS fibroblast. Arrows show autophagosomes with LC3 and cytochrome c colocalization. Colocalization of both markers was assessed by DeltaVision software using the Pearson coefficient of correlation. Bar = 5 µm. (C) Quantification of LC3 per cytochrome c puntacta in control and MELAS fibroblasts incubated with 0.06 µM riboflavin or 100 µM CoQ (n= 100 cells). *P < 0.01 between control and MELAS fibroblasts. aP < 0.01, between the presence and the absence of CoQ.