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. 2012 Nov;167(6):1311–1328. doi: 10.1111/j.1476-5381.2012.02086.x

Figure 8.

Figure 8

Effect of riboflavin and CoQ on MELAS cybrids. (A) Cell proliferation. Wt and MELAS cybrids were cultured in the absence or presence of riboflavin (0.06 µM) or CoQ (100 µM) for 72 h. Cell counting was performed as described in Methods. (B) ATP levels in Wt and MELAS cybrids in the absence or presence of riboflavin (0.06 µM) or CoQ (100 µM) for 72 h. (C) Activity of complexes I + III combined. Complexes I + III were determined as described in Methods. Results (mean ± SD) are expressed as U per CS (units per CS). (D) ROS generation in MELAS cybrids. Wt and MELAS cybrids cultures were grown in normal culture medium or in medium supplemented with riboflavin (0.06 µM) or CoQ (100 µM) for 72 h before analysis. ROS levels were then determined by flow cytometry using MitoSox red, and the mitochondrial mass was estimated using NAO Results are expressed as the ratio of MitoSOX signal to NAO signal. Data in arbitrary units (a.u.) represent the mean ± SD of three separate experiments. *P < 0.01 between Wt and MELAS cybrids. aP < 0.01 between the presence and the absence of riboflavin or CoQ.