Figure 1.

Mianserin displays higher affinity for κ- than µ- and δ-opioid receptors. (A) Radioligand binding assays were performed in membranes of CHO cells stably transfected with human κ- (CHO/KOP), µ- (CHO/MOP) and δ- (CHO/DOP) opioid receptors by using 0.2 nM [³H]diprenorphine (CHO/KOP and CHO/MOP) or 0.2 nM [³H]NTI (CHO/DOP). Non-specific binding was determined in the presence of 10 µM naloxone. Values are the mean ± SEM of three experiments. (B) Scatchard plot of [³H]diprenorphine saturation binding carried out in CHO/KOP membranes in the presence of either vehicle (control) or 10 µM mianserin (mians). Values are the mean of three experiments.