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. 2012 Nov;167(6):1329–1341. doi: 10.1111/j.1476-5381.2012.02078.x

Figure 3.

Figure 3

Stimulation of ERK1/2 phosphorylation by mianserin and mirtazapine in CHO/KOP cells. (A) Cells were incubated for the indicated time periods in the presence of 1 µM mianserin. Cell extracts were analysed for ERK1/2 phosphorylation (pERK1 and pERK2), total ERK1/2 and actin levels by Western blot. Densitometric ratios of pERK1/2 normalized for total ERK1/2 are expressed as percent of zero time values and are the mean ± SEM of four experiments. (B and C) CHO/KOP cells were incubated for 10 min in the presence of the indicated concentrations of either mianserin (mians) (B) or dynorphin A 1–13 (dyn A) (C). Values are the mean ± SEM of five and three experiments respectively. (D) CHO/KOP cells were pre-incubated in the presence of either vehicle or 100 nM nor-BNI for 10 min and then exposed to either vehicle, 1 µM mianserin or 3 µM mirtazapine (mirtaz) for 10 min. Values are the mean ± SEM of four experiments. ***P < 0.001, *P < 0.05 significantly different from control (vehicle + vehicle) by anova.