Figure 4.

Stimulation of ERK1/2 phosphorylation by mianserin and mirtazapine in C6 glioma cells. (A) Cells were incubated for 15 min in the presence of the indicated mianserin (mians) concentrations. Values are the mean ± SEM of four experiments. (B and C) C6 glioma cells were pre-incubated for 10 min with either vehicle or 100 nM nor-BNI and then exposed to either vehicle, the indicated concentrations of mianserin (B) or 3 µM mirtazapine (mirtaz; C). Values are the mean ± SEM of four experiments. ***P < 0.001, *P < 0.05 significantly different from control by anova. (D) PTX prevents the stimulation of ERK1/2 phosphorylation by mianserin and mirtazapine. C6 glioma cells were pre-incubated for 24 h with either vehicle or 100 ng·mL⁻¹ PTX and then exposed for 15 min to either 10 µM mianserin or 10 µM mirtazapine. Values are the mean ± SEM of three experiments. ***P < 0.001 significantly different from control (vehicle + vehicle) by anova.