Figure 5.

Stimulation of CREB phosphorylation by mianserin and mirtazapine. (A) CHO/KOP cells were incubated in the presence of 1 µM mianserin for the indicated time periods. Cell extracts were then analysed for phospho-Ser¹³³-CREB, total CREB and actin levels by Western blot. Values are the mean ± SEM of three experiments. (B and C) CHO/KOP (B) and C6 glioma (C) cells were pre-incubated for 10 min in the presence of either vehicle or 100 nM nor-BNI. Cells were then exposed for 20 min to either vehicle, 1 µM mianserin (mians) or 3 µM mirtazapine (mirtaz). Densitometric values are the mean ± SEM of four experiments. *P < 0.05 significantly different from control by anova.