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. 2012 Nov;167(6):1329–1341. doi: 10.1111/j.1476-5381.2012.02078.x

Figure 8.

Figure 8

Antagonism of dynorphin A stimulated p38 MAP kinase and ERK1/2 phosphorylation by mianserin in mouse primary neurons. Primary cultures of mouse striatal (A) and hippocampal (B) neurons grown for 8–10 days were treated with either vehicle or 10 µM mianserin for 10 min and then exposed to either vehicle or 30 nM dynorphin A (dyn A) for 15 min. Cell extracts were analysed for phospho-p38 MAPK (p-p38), total p38 MAPK (p38) and actin levels by Western blot. Values are the mean ± SEM of four experiments. ***P < 0.001, *P < 0.05 significantly different from control by anova. (C) Primary cultures of mouse striatal neurons were treated as indicated above and cell extracts were analysed for phospho-ERK1/2, total ERK1/2 and actin levels. Values are the mean ± SEM of four experiments. ***P < 0.001 significantly different from control by anova.