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(A) There are a total of 42 mismatched amino acids in the two published sequences for KflA (CAA71133.1 from 2000 and CAA71133.2 from 2010). Shown here are 38 of the mismatches that are mostly concentrated in three rows of the alignment of the two sequences. (B) Expression of CAA71133.2 gave soluble, active protein with the expected molecular mass (56.3 kDa theoretical for post-translationally cleaved protein).
