Table 4.

Relationships between clinical and endoscopic variables, standard and measured histopathological variables, immunohistochemical results and lymph node metastasis in the 170 tissue microarray cases with submucosal invasion thickness ≥250µm

Parameters		Total	Lymph node metastasis (%)	Univariate Logistic regression				Receiver-Operating-Characteristic curve
				Odds ratio	95% CI	Global P	P- for-trend	Area under the curve	95% CI	P
Clinical variables
Sex	Male	124	38(31)	1				0.555	0.460–0.649	0.271
	Female	46	9(20)	0.550	0.242–1.253	0.155
Age	<60	106	32(30)	1				0.540	0.444–0.636	0.425
	≥60	64	15(23)	0.708	0.347–1.442	0.341
Symptoms	No	5	0	1				0.520	0.425–0.616	0.682
	Yes	165	47(28)	6.435×108	0-∝	0.999
Endoscopic variables
Tumor location	Cervical	2	0	0	0-∝	0.999		0.578	0.481–0.675	0.117
	Upper thoracic	33	7(21)	0.736	0.289–1.872	0.520
	Middle thoracic	112	30(27)	1
	Lower thoracic	23	10(43)	2.103	0.834–5.299	0.115
Tumor size (measured	<2cm	37	8(22)	1				0.533	0.437–0.628	0.509
endoscopically)	≥2cm	133	39(29)	1.504	0.632–3.580	0.356
Macroscopic type	Papillary	22	2(9)	1				0.618	0.527–0.710	0.017
	Erosive	32	7(22)	2.800	0.523–14.992	0.229
	Plaque-like	92	28(30)	4.375	0.957–20.001	0.057
	Ulcerative	10	5(50)	10.000	1.480–67.554	0.018
	Intraluminal	14	5(36)	5.556	0.901–34.246	0.065
	mass
Standard histopathological variables
Invasion depth level	Sm1	10	3(30)	1.423	0.766–2.646		0.264	0.556	0.460–0.651	0.262
	Sm2	41	7(17)
	Sm3	119	37(31)
Degree of differentiation	Well	36	4(11)	1				0.617	0.526–0.707	0.019
	Moderate	68	21(31)	3.574	1.121–11.400	0.031
	Poor	40	14(35)	4.308	1.264–14.676	0.020
	Basaloid	19	5(26)	2.857	0.665–12.267	0.158
	Sarcomatoid	7	3(43)	6.000	0.970–37.121	0.054
Angiolymphatic invasion	No	132	32(24)	1				0.566	0.467–0.665	0.183
	Yes	38	15(39)	2.038	0.951–4.369	0.067
Multicentric invasive	No	160	39(24)	1				0.518	0.420–0.617	0.714
lesions	Yes	10	8(80)	1.814	0.488–6.740	0.374
Glandular involvement by	No	129	33(26)	1				0.539	0.441–0.638	0.430
high-grade dysplasia/	Yes	41	14(34)	1.508	0.708–3.216	0.287
carcinoma-in situ
Measured histopathological variables
Tumor thickness	<3000µm	34	6(18)	1				0.550	0.456–0.644	0.314
	≥3000µm	136	41(30)	2.014	0.775–5.233	0.151
Submucosal invasion	<2000µm	71	14(20)	1				0.583	0.489–0.677	0.095
thickness
	≥2000µm	99	33(33)	2.036	0.992–4.176	0.053
Diameter of the largest	<2cm	90	25(28)	1				0.508	0.411–0.606	0.867
invasive lesion
	≥2cm	80	22(28)	0.935	0.476–1.838	0.846
Immunohistochemical staining
P53	−	76	19(25)	1.100	0.739–1.637		0.638	0.526	0.430–0.622	0.606
	+	41	15(37)
	++	47	13(28)
CyclinD1	−	51	12(24)	1.155	0.766–1.740		0.492	0.531	0.434–0.628	0.534
	+	47	15(32)
	++	64	19(30)
EGFR	−	59	14(24)	1.506	0.984–2.304		0.060	0.589	0.487–0.691	0.079
	+	53	10(19)
	++	52	21(40)
VEGF	−	51	7(14)	2.133	1.362–3.340		0.001	0.660	0.570–0.750	0.001
	+	45	12(27)
	++	66	28(42)

Abbreviation: CI, confidence interval.