Figure 1.

Methodology Comparison.A. Allelic discrimination plots depicting genotyping results for the same samples using either purified or unpurified DNA. The left panel shows genotyping directly from blood and the right panel shows genotyping after purification. The wild type allele used the VIC probe (y-axis) while the minor allele used the FAM probe (x-axis). B. The variance between samples was assessed by calculating the standard deviation of the wild type clusters for all of the assays. The results for genotyping directly are shown in blue and after purification are shown in red.