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Western blot detection of encoded proteins secreted from COS-7 cells transfected with DNA vaccine vectors. Lane 1, VR1012 transfected cell supernatant; lane 2, pre-stained protein marker; lane 3, VR1012 Cat B2 cell supernatant; lane 4, VR1020 Cat B2 cell supernatant; lane 5, MCP3 Cat B2 cell supernatant; lane 6, CTLA4 Cat B2 cell supernatant; lane 7, yeast expressed Cat B as a positive control.
