Table 2.
DGE sequencing statistics
| |
SG |
S-lab |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
Egg |
3rd Larva |
Pupa |
Adult |
3rd Larva |
|||||
| N | % | N | % | N | % | N | % | N | % | |
| Total raw tag |
3762501 |
|
3790500 |
|
3524500 |
|
3769500 |
|
3727501 |
|
| Total clean tag |
3759150 |
|
3787346 |
|
3521826 |
|
3734683 |
|
3724228 |
|
| Distinct clean tag |
156922 |
|
118011 |
|
132376 |
|
113095 |
|
143277 |
|
| Distinct tag mapping to gene |
59206 |
37.7 |
42706 |
36.2 |
45666 |
34.5 |
41254 |
36.5 |
51264 |
35.8 |
| Distinct unambiguous tag mapping to gene |
48378 |
30.8 |
34772 |
29.5 |
37693 |
28.5 |
34449 |
30.5 |
39475 |
27.6 |
| Unambiguous tag-mapped genes |
10917 |
53.8 |
9808 |
48.3 |
10252 |
50.5 |
9759 |
48.1 |
10976 |
54.1 |
| Distinct tag mapping to genome |
48679 |
31.0 |
36533 |
31.0 |
40063 |
30.3 |
35062 |
31.0 |
47035 |
32.8 |
| Total unknown tag |
424236 |
11.3 |
397001 |
10.5 |
442991 |
12.6 |
415816 |
11.1 |
355720 |
9.6 |
| Distinct unknown tag | 49037 | 31.2 | 38772 | 32.9 | 46647 | 35.2 | 36779 | 32.5 | 44978 | 31.4 |
For each number (N) of tags or tag-mapped genes, the associated percentage (%) regarding the distinct clean tags (156922), the reference genes (20306), or total clean tags (3759150) is indicated.