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(a) The uptake of TF1-antiCD4 and TF2-antiCD19 antibodies into the covalently immobilized microgels onto smart platform was analyzed by detecting the fluorescence arising from the two TFs fluorophores. (b) Fluorescence spectra of the immobilized microgels after the uptake process (dotted line), assessing the location of both fluorescent biomolecules inside the immobilized microparticles, and after release of labeled antibodies (straight line). The scale bar was 5 μm.
