Fluorescence micrographs of N-terminal CB1 immunoreactivity in the brain 24 h after saline or LPS treatment (1 mg/kg, i.p.). Dense fine fiber immunoreactivity was distributed in forebrain, especially cortex (image captured from cingulate and motor cortex) (A, B), hippocampus (Hipp; C, D) and basolateral nucleus of amygdala (Amyg; E, F). Immunoreactive neurons (inset in panel E), were also observed within these regions. In brainstem, there was some weak immunoreactivity found around the 4th ventricle (G, H) and at the margin of hypoglossal nucleus adjoining the dorsal motor nucleus of the vagus (Hyp). However, no immunoreactivity was observed in CVOs (I & J, vascular organ of the lamina terminalis [OVLT]; K & L, subfornical organ [SFO]; M & N, median eminence [MBH]; O & P, area postrema [AP]). Scale bars: 100 µm (A – F, I - P), 50 µm (G, H and inset in Panel E).