Table 2.

Influence of restricted feeding on multiple energy balance parameters. Mice were fed standard laboratory chow in an ad libitum (Ad Lib; n=44) fashion, followed by random assignment to dark phase (DP; n=21) or light phase (LP; n=23) restricted feeding groups. All parameters were monitored continuously using a CLAMS; restricted feeding was enforced in an automated manner by a CLAMS. Data for food intake (g) and activity (beam breaks) are totals (i.e., the sum during a distinct time period), while data for energy expenditure (kcals/hr) and RER are averages (i.e., the average during a distinct time period). Data are reported as mean ± SEM.

	12h Light Period Total/Average			12h Dark Period Total/Average			24h Daily Total/Average
	Ad Lib	LP	DP	Ad Lib	LP	DP	Ad Lib	LP	DP
Food Intake	1.53 ± 0.05	3.97 ± 0.08*	0.00 ± 0.00*$	2.30 ± 0.05	0.00 ± 0.00*	3.60 ± 0.07*$	3.83 ± 0.08	3.97 ± 0.08	3.60 ± 0.07$
Energy Expenditure	0.34 ± 0.01	0.38 ± 0.01*	0.29 ± 0.01*$	0.45 ± 0.01	0.35 ± 0.01*	0.46 ± 0.01$	0.39 ± 0.01	0.36 ± 0.01*	0.38 ± 0.01
RER	0.93 ± 0.01	1.09 ± 0.01*	0.84 ± 0.01*$	0.96 ± 0.00	0.90 ± 0.01*	1.05 ± 0.00*$	0.95 ± 0.00	0.99 ± 0.00*	0.94 ± 0.00$
Activity	7638 ± 500	6337 ± 586	8068 ± 822	12811 ± 764	13748 ± 938	14805 ± 997	20449 ± 1178	20084 ± 1352	22874 ± 1717

^*p<0.05 compared to Ad Lib fed conditions (paired t-test);

^$p<0.05 for DP versus LP fed mice (unpaired t-test).