Table 3.
Influence of restricted feeding on the phase and amplitude of diurnal variations in expression of circadian clock genes within metabolically active peripheral tissues. Mice were randomly assigned to dark phase (DP; n=21) or light phase (LP; n=23) restricted feeding groups. Data are reported as mean ± SEM. Phase is the zeitgeber time (ZT; in hours) at which the peak value in the oscillation occurs. Amplitude (i.e., peak-to-trough difference) units are mRNA molecules per ng total RNA. NS signifies not significant.
| Parameter | Tissue | Feeding Group | Gene | ||||
|---|---|---|---|---|---|---|---|
| bmal1 | per2 | cry2 | rev-erbα | dbp | |||
| Phase | Liver | LP | 13.66±0.39 | 4.33±0.34 | 3.18±0.98 | 23.80±1.07 | 1.53±0.78 |
| DP | 22.02±0.55$ | 15.08±0.50$ | 12.2±0.57$ | 6.03±0.88$ | 9.05±0.53$ | ||
| Epididymal Fat | LP | 12.80±1.12 | 10.82±1.54 | NS | 2.05±1.63 | 4.27±1.03 | |
| DP | 22.85±0.58$ | 13.48±0.70 | NS | 6.10±1.17 | 9.49±1.08$ | ||
| Gastrocnemius Muscle | LP | 18.18±1.5 | NS | NS | 6.98±1.65 | 5.28±0.78 | |
| DP | 22.78±0.89$ | 13.87±1.52 | NS | 5.82±1.63 | 9.89±0.62$ | ||
| Heart | LP | 19.29±1.02 | 9.25±0.90 | NS | 4.02±0.66 | 5.14±0.62 | |
| DP | 0.26±0.55$ | 12.54±0.64$ | 12.94±0.70 | 6.18±0.77$ | 10.31±0.75$ | ||
| Amplitude | Liver | LP | 449.76±46.58 | 211.38±19.02 | 494±126.88 | 126.1±35.34 | 66354±13698 |
| DP | 528.26±78.34 | 191.34±25.14 | 687.54±107.52 | 131.96±29.2 | 67042±9340 | ||
| Epididymal Fat | LP | 399.06±116.96 | 34.8±14.12 | NS | 90.2±38.64 | 2860±774 | |
| DP | 1248.78±198.84$ | 96.94±18.4$ | NS | 419.54±123.7$ | 7964±2286$ | ||
| Gastrocnemius Muscle | LP | 258.3±101.82 | NS | NS | 48.24±20.94 | 1827.94±361.76 | |
| DP | 602.06±146.56 | 10.96±5.18 | NS | 40.54±14.66 | 2690.96±460.04 | ||
| Heart | LP | 115.78±31.16 | 1045.28±249.08 | NS | 4544±748 | 9026±1480 | |
| DP | 314.86±47.88$ | 2743.8±487.54$ | 243.46±47.04 | 9200±1788$ | 15100±3090 | ||
$
p<0.05 for DP versus LP fed mice.