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. Author manuscript; available in PMC: 2013 Dec 27.
Published in final edited form as: Neuroscience. 2012 Sep 25;227:44–54. doi: 10.1016/j.neuroscience.2012.09.043

Figure 2. Residues within the RNA recognition motif of TDP-43 mediate its interaction with NAC1.

Figure 2

LNAC1 and TDP-43 N-terminus truncations, designated as 187, 197, and 1–198 (1–198 AA, 187–414 AA, and 197–414 AA) proteins were synthesized in vitro using S35-labeled methionine. TDP-43 truncations proteins were incubated with glutathione sepharose beads bound with GST-lNAC1 protein and confirm that the LNAC1 show protein–protein interaction with the TDP-43 truncations proteins tested. No band was observed with GST alone. Lanes 1, 2, 3, and 4 are IVT products, lane 5 is a positive control (GST−POZ+LNAC1), lane 6, 8, 10 are negative controls, and lanes 7, 9, 11 are test groups. The schematic below the blot shows the functional domains of the TDP-43 protein in each of the truncated variants.