Skip to main content
. Author manuscript; available in PMC: 2013 Dec 27.
Published in final edited form as: Neuroscience. 2012 Sep 25;227:44–54. doi: 10.1016/j.neuroscience.2012.09.043

Figure 7. Cytoplasmic TDP-43 co-localizes with NAC1, and 19S following nucleo-cytoplasmic translocation.

Figure 7

(A–C) In untreated neurons localization of TDP-43 was restricted to the nucleus whereas NAC1 expression was mainly nuclear with some cytoplasmic staining. (D–F) After glutamate treatment TDP-43 is translocated from the nucleus into the cytoplasm, with TDP-43 staining extending out into distant neuronal processes. (G–I) High magnification images of inset from panel F show glutamate-induced increased cytoplasmic co-localization of NAC1 and TDP-43, most easily seen in dendtrites (white arrows). (J–L) In untreated neurons, localization of the 19S subunit of the proteasome was mainly nuclear. (M–O) Following glutamate treatment, 19S and TDP-43 were translocated out of the nucleus into the cytoplasm. (P–R) High magnification images of inset from panel O show co-translocated cytoplasmic 19S and TDP-43, which was especially obvious in the proximal dendrites (white arrows).