Figure 1.

Effect of Triton X-100 on SS recovery and activity. A, SS levels of amyloplast stromal fractions. Amyloplasts were released from endosperm, as described in Methods and recovered by low-speed centrifugation. Stromal fractions were generated by suspension of amyloplasts in buffer A containing Triton X-100 at the levels indicated. Suspensions were mixed and centrifuged. Stromal fractions were recovered and assayed for SS activity. B, Effect of Triton X-100 on AGP (▴), SBE (•), and SS (□) activities of a soluble endosperm extract. Endosperm extracts were prepared as described in Methods and aliquots were assayed at the indicated levels of Triton X-100.