Figure 3.

Immunoblots of endosperm and amyloplast stromal extracts probed with ADH, SSI, SBEIIb, and SU1 antibodies. SDS gels were run with amyloplast stromal fractions (Am), soluble extracts from whole endosperm (En), or SDS extracts corresponding to 2.5 mg of isolated starch granules (Gr). Immunoblots were probed with antibodies recognizing ADH (A), SSI (B), SBEIIb (C), and SU1 (D). The blots shown in A and D were visualized by electrochemiluminescence. The blots shown in B and C were visualized colorimetrically. Each lane contained 5 μg of protein.