FIG. 6.

Barrier TEF2-UAS coincides with nuclease-hypersensitive chromatin. (A) TEF2-UAS counteracts HML and telomeric silencing. Strain H23 has TEF2-UAS consisting of three tandem Rap1p sites (arrows) inserted between HML and URA3. TEF2-UAS was inserted between TEL-VII-L and URA3 in T15. The growth phenotypes of H0, H23, T1, and T15 are shown. (B) TEF2-UAS can block the spread of silent chromatin. The results of ChIP performed on H0 and H23 using α-Sir3p are shown. (C) Special chromatin structure associated with TEF2-UAS. The results of indirect end labeling on strains H0 and H23 are shown. MNase-treated DNA was digested with AvaII.