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. 2012 Nov 7;32(45):15934–15945. doi: 10.1523/JNEUROSCI.3227-12.2012

Figure 3.

Figure 3.

Temporally restricted CPEC competency correlates with NEC rather than RG fate. A, SFEBq aggregates treated with BMP4 for 7 DIV (M1) or 5 DIV (M2) (qRT-PCR normalized to no BMP4 controls). Five-day M1 and M2 aggregates show much stronger CPEC gene induction than 7-, 9-, or 11-day aggregates. B–E, RC2 ICC of sectioned M1 and M2 aggregates. Five-day aggregrates exhibit low RC2 expression levels characteristic of NECs, while strong RC2 expression typical of RG is present throughout 7-day aggregates. F–G, Sox1 and Nestin ICC of sectioned M2 aggregates. Five-day aggregates exhibit the strong Sox1 and weak Nestin patterns characteristic of NECs, whereas 7-day aggregates display the opposite RG-like pattern. H, Scoring of Sox1/Nestin staining patterns in M2 aggregates. Scale bars, 100 μm.