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. 2012 Nov 9;99(12):985–998. doi: 10.1007/s00114-012-0976-3

Table 2.

Results of the behavioral experiment

Species Examined Solutions
10 mM 10 mM 10 mM 10 mM 1 mM 100 mM 1 M 10 mM 10 mM 10 mM
NaCl KCl CaCl2 MgCl2 NaCl NaCl NaCl NaNO2 NaHCO3 NaH2PO4
P. machaon (0, 0, 2) (1, 0, 0) (1, 0, 0) (1, 0, 0) (1, 0, 0) (0, 0, 1) (0, 1, 0) (0, 0, 2) (0, 0, 1) (0, 0, 1)
P. xuthus (0, 0, 5) (2, 0, 0) (3, 0, 0) (4, 0, 0) (0, 2, 1) (1, 1, 2) (0, 1, 0) (0, 0, 5) (0, 0, 5) (0, 0, 5)
P. xuthus (0, 0, 1) (0, 1, 0) (0, 0, 1) (1, 0, 0)
P. maackii (0, 0, 28) (18, 2, 3) (18, 2, 1) (20, 0, 0) (3, 6, 11) (0, 6, 13) (4, 9, 5) (2, 0, 21) (0, 1, 22) (0, 1, 22)
P. bianor (0, 0, 24) (23, 2, 0) (21, 4, 1) (21, 3, 0) (4, 7, 17) (4, 9, 16) (4, 15, 2) (0, 0, 25) (0, 1, 26) (0, 1, 25)
P. helenus (0, 0, 15) (12, 0, 0) (8, 2, 0) (13, 0, 0) (2, 2, 11) (1, 0, 13) (2, 8, 5) (0, 1, 8) (0, 0, 11) (0, 0, 11)
P. helenus (0, 0, 1) (1, 1, 0) (1, 1, 0) (0, 1, 0) (0, 0, 1) (0, 1, 0) (0, 0, 1) (0, 1, 0) (0, 0, 1)
P. protenor (0, 0, 11) (10, 3, 0) (7, 5, 0) (10, 2, 0) (2, 4, 5) (0, 3, 8) (2, 7, 0) (0, 0, 10) (0, 0, 10) (0, 1, 8)
P. protenor* (0, 0, 1)
P. macilentus (0, 0, 13) (8, 0, 0) (8, 1, 0) (10, 1, 0) (2, 4, 5) (0, 0, 10) (2, 7, 1) (0, 0, 8) (0, 0, 10) (0, 0, 12)
P. memnon (1, 0, 1) (1, 0, 0) (1, 0, 0) (2, 0, 0) (2, 0, 0) (0, 2, 1) (1, 1, 0) (0, 0, 1) (0, 0, 2) (0, 0, 2)
P. memnon (0, 0, 1) (0, 1, 0) (0, 1, 0) (1, 0, 0)
P. memnon †† (0, 0, 1)
Total (1, 0, 102) (76, 10, 3) (68, 16, 3) (87, 7, 0) (16, 25, 50) (6, 21, 65) (15, 50, 13) (2, 1, 81) (0, 3, 87) (0, 3, 87)

Puddling scores of eight species of 212 male Papilio butterflies and a female P. protenor in response to test solutions in puddling trays. Numerical values in (a, b, c) are as follows: a, the number of butterflies that were estimated as showing a “score 1” behavior to a corresponding test solution; b, the number of butterflies that were estimated as demonstrating a “score 2” behavior to a corresponding test solution; c, the number of butterflies that were estimated as showing a “score 3” behavior to a corresponding test solution. The scores were high for puddling behaviors to the NaCl solutions in all species of butterflies

xuthus ”; “helenus ” and “memnon ” indicate that these butterflies were kept in the Toyosato insect Museum Green House

memnon ††” indicate that this butterfly was kept in the Adachi Park of living things, Tokyo

All other results were obtained in the field

protenor*” indicates a female P. protenor