Fig. 2.

Neural crest related markers are expressed upon retinoic acid mediated differentiation of ESCs. (a) RA treated or untreated EBs were immunohistochemically analysed for p75, AP2α, Pax3, Pax6 and Vimentin 1 day after dissociation. Though in untreated cells Vimentin expression was detectable in form of non-filamentous cluster (inset), Vimentin filaments were only detectable in the minority of the cells (indicated by arrowheads). Scale bar: 100 μm. (b) 5 days after dissociation of RA treated or untreated EBs, cells were labeled for SMA and Tuj1. Scale bar: 200 μm. RA was applied after 4 and 6 days of EB formation as indicated. Dissociation of EBs was performed after 8 days of cultivation in suspension. For quantification of immunohistochemical analysis >300 cells were evaluated. Data indicate Mean ± SD