Table 1.
Gene | Primers | Product size (bp) | Accession number |
---|---|---|---|
MuRF1 | Forward: 5′GGT GCC TAC TTG CTC CTT GT3′ | 203 | AY059627.1 |
Reverse: 5′TCA CCT GGT GGC TGT TTT C3′ | |||
MAFbx | Forward: 5′TTA TGC ACG CTG GTC CAG A3′ | 170 | NM_133521 |
Reverse: 5′TGT AAG CAC ACA GGC AGG TC3′ | |||
UCP2 | Forward: 5′-ACA AGA CCA TTG CAC GAG AG-3′ | 293 | AF039033 |
Reverse: 5′-CAT GGT CAG GGC ACA GTG GC-3′ | |||
UCP3 | Forward: 5′GTG ACC TAT GAC ATC ATC AAG GA3′ | 89 | NM_013167 |
Reverse: 5′GCT CCA AAG GCA GAG ACA AAG3′ | |||
β-Actin | Forward: 5′CTG GCT CCT AGC ACC ATG AA3′ | 103 | NM_031144 |
Reverse: 5′CTG CTT GCT GAT CCA CAT CT5′ |
MAFbx Muscle Atrophy F box or Atrogin 1, MuRF1 Muscle RING Finger 1, UCP Uncoupling Protein 2 and 3