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. 2012 May 31;3(4):265–275. doi: 10.1007/s13539-012-0071-9

Table 1.

Oligonucleotide primer sequences used for qPCR analysis

Gene Primers Product size (bp) Accession number
MuRF1 Forward: 5′GGT GCC TAC TTG CTC CTT GT3′ 203 AY059627.1
Reverse: 5′TCA CCT GGT GGC TGT TTT C3′
MAFbx Forward: 5′TTA TGC ACG CTG GTC CAG A3′ 170 NM_133521
Reverse: 5′TGT AAG CAC ACA GGC AGG TC3′
UCP2 Forward: 5′-ACA AGA CCA TTG CAC GAG AG-3′ 293 AF039033
Reverse: 5′-CAT GGT CAG GGC ACA GTG GC-3′
UCP3 Forward: 5′GTG ACC TAT GAC ATC ATC AAG GA3′ 89 NM_013167
Reverse: 5′GCT CCA AAG GCA GAG ACA AAG3′
β-Actin Forward: 5′CTG GCT CCT AGC ACC ATG AA3′ 103 NM_031144
Reverse: 5′CTG CTT GCT GAT CCA CAT CT5′

MAFbx Muscle Atrophy F box or Atrogin 1, MuRF1 Muscle RING Finger 1, UCP Uncoupling Protein 2 and 3