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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Nat Protoc. 2012 Jan 12;7(2):235–246. doi: 10.1038/nprot.2011.437

Figure 3.

Figure 3

Detection of side population of esophageal epithelial cells. (a) Forward (FSC-A) and side (SSC-A) scatter of primary esophageal epithelial cells. Single cells are gated (usually 200–400 FCS-A and 100–400 SSC-A). (b) Live cells gated as PI intensity below 101.3 (PI was excited at 488 nm and its emission was measured in a logarithmic scale through a band-pass filter of 630/22A). (c) Verapamil-treated cells show diminished side population fraction. (d) Side population cells (tail of cells with Hoechst red below 300 and Hoechst blue below 400) and side population–negative cells (Hoechst red above 300, Hoechst blue over 400). Modified from ref. 23 with permission.