TABLE 2.
Antibodies validated in immunostaining of sections from OTC.
| Protein | Primary antibody | Titer and other conditions | Company (cat. no.) | Recognizes | Isotype | Secondary antibody |
|---|---|---|---|---|---|---|
| CK14 | Rabbit anti-CK14 | (1:3,000) Microwave at pH 6.0 | Covance (MK14 PRB155p) | Human, mouse | IgG | IHC, IFa |
| CK13 | Mouse anti-CK13 clone 2D7 | (1:250) Microwave at pH 6.0 | Abcam (ab90096) | Human, mouse, rat | IgG2b | IHC, IFb |
| Ki-67 | Rabbit anti-Ki67 | (1:1,000) Microwave at pH 6.0 | Novocastra/Leica (NCL-Ki67p) | Human | IgG | IHC, IFa |
| Involucrin | Rabbit anti-involucrin | (1:100) Microwave at pH 6.0 | Covance (PRB 140C) | Mouse | IgG | IHC, IFa |
| Involucrin | Mouse anti-involucrin clone SY5 | (1:750) Microwave at pH 6.0 | Sigma-Aldrich (I9018) | Human | IgG1 | IHC, IFb |
IF, immunofluorescence; IHC, immunohistochemistry.
a
IHC: biotinylated anti-rabbit IgG (H + L), Vector BA-1000, 1:200; IF–cy3 donkey anti-rabbit IgG (H + L), Jackson ImmunoResearch Lab. no. 711–165–152, 1:600.
b
IHC: biotinylated anti-mouse IgG (H + L), rat absorbed, Vector BA-1000, 1:200; IF–cy3 donkey anti-mouse IgG (H + L) Jackson ImmunoResearch Lab. no. 711–165–150, 1:600.