50-ml tube ‘A’ acellular layer components	Transwell carriers
	× 1 (6 Inserts)	× 2 (12 Inserts)
10× EMEM	690 μl	1,380 μl
FBS	700 μl	1,400 μl
L-Glutamine	60 μl	120 μl
Sodium bicarbonate	140 μl	280 μl
Bovine collagen type I	5.6 ml	11.2 ml