50-ml tube ‘C’ cellular layer components	Transwell carriers
	× 1 (6 Inserts)	× 2 (12 Inserts)
10× EMEM	1.8 ml	3.6 ml
FBS	2 ml	4 ml
L-Glutamine	160 μl	320 μl
Sodium bicarbonate	380 μl	760 μl
Bovine collagen	11.4 ml	22.8 ml
Matrigel	3.8 ml	7.6 ml
6 × 105 per ml fibroblasts	1.6 ml	3.2 ml