For two Transwell carriers (12 wells; two medium changes)	EPM1 (300 ml), store at 4 °C	EPM2 (200 ml), store at 4 °C
DMEM	218 ml	95 ml
Ham’s F12	72 ml	95 ml
L-Glutamine	6 ml	4 ml
Hydrocortisone (H)	600 μl	400 μl
ITES	600 μl	400 μl
O-Phosphorylethanolamine (O)	600 μl	400 μl
Adenine (A)	600 μl	400 μl
Progesterone (P)	600 μl	—
Triiodothyronine (T)	600 μl	400 μl
NBCS	300 μl	4 ml
Gentamicin sulfate	300 μl	200 μl