FIG. 7.

Differential requirements of multiple ARCs for telomere elongation by tankyrase 1. (A) Effects of overexpression of FN-tankyrase 1 constructs on telomere length in HTC75 cells. TRF at the indicated PD were detected by Southern blot analysis. The results of two representative experiments are shown. M, mock; WT-Tank, wild-type FN-tankyrase 1; mt, mutant. (B) Graphic representations of telomere length change. The plots represent the mean TRF values derived the results presented in panel A. (C) Western blot analysis of whole-cell or nuclear extracts (20 μg of proteins per lane). Blots were probed with antibodies as indicated. For detection of self-poly(ADP-ribosyl)ation, lysates were immunoprecipitated (IP) with anti-FLAG antibody and the pellets were subjected to Western blot analysis with anti-poly(ADP-ribose). MW, molecular mass.