FIG. 7.

Filter hybridization of RNA from Lmo4 mutant embryos. Total RNA was extracted from E12.5 embryos with normal and mutant phenotypes, fractionated on a 1.4% agarose gel, transferred to a nylon membrane, and hybridized to a probe containing Lmo4 cDNA sequences 3′ to the neo insertion site in exon 2 (see Fig. 5). RNA loading was monitored by reprobing the filter with a mouse β-actin cDNA. RNA of representatives from wild-type (wt), Lmo4^+/−, and Lmo4^−/− exencephalic or nonexencephalic embryos.