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. 2004 Mar;24(5):1918–1929. doi: 10.1128/MCB.24.5.1918-1929.2004

FIG. 10.

FIG. 10.

Expression of total proteins and phosphoproteins of p42/44 MAPK (A) and p38 MAPK (B). Wild-type, IRS-1 KO, IRS-2 KO, IRS-3 KO, and IRS-4 KO cells and IRS-1 KO cells reconstituted with (+) IRS-1, IRS-4, or the N1.C2 chimera were differentiated, and protein lysates were prepared on days 0, 2, and 6 of differentiation. The lysates were separated by sodium dodecyl sulfate-0% polyacrylamide gel electrophoresis and analyzed by Western blotting using antibodies against p44/42 MAPK and p38 MAPK (left) or phospho-p44/42 MAPK and phospho-p38 MAPK (right).