Skip to main content
. 2004 Mar;24(5):2052–2062. doi: 10.1128/MCB.24.5.2052-2062.2004

FIG. 5.

FIG. 5.

PC4 stimulates p53- and p53Δ30-mediated reporter gene expression in vivo. (A) H1299 (p53 null) cells were transiently transfected with the mammalian expression constructs of p53 (250 ng) or PC4 (2.5 μg) either alone or in combination. The p53-responsive promoter construct pG13Luc (1 μg) was used to measure the luciferase activity driven by p53. (B) H1299 cells were cotransfected with p53 (250 ng) or PC4 (4.5 μg) either alone or in combination. The reporter construct used was MDM2Luc (1 μg). (C) p53Δ30 (100 ng) or PC4 (2.5 μg) was cotransfected either alone or in combination with PG13Luc. (D) H1299 cells were cotransfected with p53Δ30 (100 ng) or PC4 (4.5 μg) either alone or in combination. MDM2Luc (1 μg) was used as the reporter construct. Equivalent amounts of control empty vectors were used to normalize the amount of transfected DNA. pCMV-βgal (1 μg) was used as an internal control for all the transfection experiments to normalize luciferase activity. After normalization, relative luciferase activity was plotted (y axis). The relative luciferase activity is represented as an average of triplicate samples.