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PC4 stimulates p53-dependent Bax expression. H1299 cells were transfected with p53 (5 μg) and p53Δ30 (2.5 μg) either alone or in combination with PC4 (20 μg). After 48 h cells were lysed and total cellular proteins were subjected to SDS-PAGE followed by Western blotting with antibodies against Bax, p53, p53Δ30, PC4, and actin.
