FIG. 5.

Synergistic coactivator function between Fli-I and CARM1. CV-1 cells were transfected with MMTV(TRE)-LUC (250 ng) (A and B) or MMTV(ERE)-LUC (250 ng) (C) and expression vectors encoding TR (1 ng) or ER (1 ng), GRIP1 (250 ng), CARM1 wild type or E267Q mutant (500 ng), PRMT1 (500 ng), p300 (500 ng), and full-length Fli-I or the LRR or gelsolin-like fragment (500 ng). (A) Full-length Fli-I required for synergy. (B) Fli-I synergy with CARM1 but not with PRMT1 or p300. (C) Methyltransferase activity of CARM1 required for synergy with Fli-I. Transfected cells were grown with T3 (A and B) or E2 (C) before harvest. Results in each panel are representative of three or more independent experiments.