TABLE 1.
Summary of Antibodies used for Immunohistochemical Analysis
| Vendor | Clone | Pre-treatment | Dilution | |
|---|---|---|---|---|
| PTEN | Cell Signaling | D4.3 | EDTA, 45 min | 1:50 |
| c-MYC | Epitomics | Y69 | EDTA, 45 min | 1:300 |
| p27 | Transduction Lab | 57 | Citrate, 25 min | 1:4000 |
| phos-AKT | Cell Signaling | 736E11* | EDTA, 45 min | 1:50 |
| phos-S6 | Cell Signaling | Polyclonal** | EDTA, 45 min | 1:200 |
| 4EBP-1 | ProSci | Polyclonal | Citrate, 25 min | 1:250 |
| HIF-1α | Novus Biologicals | NB100–123 | Heat (oven) at 62C, 60 min | 1:1600 |
*
Phosphorylation site at Ser473;
**
Phosphorylation site at Ser235/236