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Signaling pathway inhibition of human ES cell-derived cardiomyocyte proliferation. H7 human ES cells were differentiated for 19 days before Percoll separation, and fraction IV cells were cultured for an additional 4 days in serum-free media with various signaling inhibitors. The inhibitors were added for the final 48 hours of culture prior to fixation. Only the PI 3-kinase inhibitor (LY294002) significantly inhibited human ES cell-derived cardiomyocyte proliferation (* = P < 0.05).
