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Stimulation of human ES cell-derived cardiomyocyte proliferation. H7 human ES cells were differentiated for 17 days before Percoll separation, and fraction IV cells were cultured for an additional 4 days in serum-free media. Various concentrations of recombinant human IGF-1 or IGF-2 were added for the final 48 hours of culture prior to fixation. IGFs increased cardiomyocyte proliferation in a dose-dependent manner to levels greater than treatment with 20% FBS (* = P < 0.05).
